Figure 1.

Host, graft and exogenous ECs contribute to revascularization of transplanted ovarian tissue. (a) Ovaries from VEGFR2-GFP mice were transplanted into RFP host; following two weeks, mice were injected with GIB4 lectin to label functional vessels and grafts were isolated to determine transit of host and graft ECs. (b) Ovaries from RFP mice were transplanted into VEGFR2-GFP host; following two weeks, grafts were isolated to assess infiltration of host vessels. (c) Ovaries were isolated from wild-type mice and co-transplanted with GFP⁺ ExECs derived from mouse heart; following two weeks mice were injected with GIB4 lectin to label functional vessels and grafts were isolated to assess contribution of ExECs to functional vessels. (d) Out of all GFP⁺ ExEC area, the percentage of Lectin⁺ area was measured. (e) Out of all Lectin⁺ area, the percentage of GFP⁺ ExEC area was measured. Insets in (a–c) are enlarged in the associated box. Error bars in (d and e) represent standard deviation between 10 representative tissue sections from 2 mice replicates. Scale bars = 100 μm.