Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 15;7:8299. doi: 10.1038/s41598-017-08266-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Tracing of the contribution of sister two-cell stage blastomeres to epiblast. Each blastomere in two-cell embryos were microinjected with fluorescent dextran beads of different colors (OGDB, OY568DB), followed by in vitro culture to blastocyst and NANOG immunofluorescence analysis (A). The quality of the microinjections was validated by survival rates of the blastomeres, blastocyst rates, and total cell numbers of blastocysts (B). The relative contribution of each two-cell blastomere to EPI (ratio from 0.5 to 1) and the absolute total number of EPI cells (•) are shown for non-bisected (C) and twin (D) embryos. Error bars in (B) are standard deviations. Black and gray bars in (C) and (D) indicate embryos cultured as pools and embryos cultured individually, respectively.