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. 2017 Aug 15;7:8293. doi: 10.1038/s41598-017-08963-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological changes of the HaCaT or MCF-7 cell membranes after L-K6 treatment. The HaCaT (a) or MCF-7 (b) cells were cultured and treated with 50 μM L-K6 for 30 min. The membrane fluorescence image indicated that while the HaCaT cell membrane showed no significant morphological changes following 30 min of L-K6 exposure, dramatic morphological changes were observed in the MCF-7 cell membrane, as indicated by cytoplasmic membrane perturbations and blebbing [yellow arrowheads; (c) pre-treatment; (d) post-treatment]. In addition, the dynamic tracing indicated that these morphological changes occurred as early as 10 min after L-K6 exposure (e).