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. 2017 Aug 15;7:8202. doi: 10.1038/s41598-017-08392-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of sustained ciprofloxacin exposure on Jurkat cells. (a,b) Viability of Jurkat cells with/without exposure to ciprofloxacin (10 µg/mL for >14 days) was analyzed by flow cytometry after staining with annexinV-FITC and propidium iodide (PI). (a) Mean values+/− S.D. (error bars) of two independent experiments are shown in the histogram plot. AxV: annexinV. (b) Representative dot plots showing the four quadrants of annexinV-FITC and PI stained Jurkat cells. (c) Exosomes (EXOs) derived from ciprofloxacin-exposed/unexposed Jurkat cells were conjugated onto latex beads and characterized by flow cytometry after staining with annexinV-FITC, anti-CD63-PE, PI or anti-histone H2B-FITC. The background fluorescence of stained latex beads is indicated by grey histograms. (d) Microvesicles (MVs) and apoptotic bodies (APOs) were labeled directly with annexinV-FITC and PI. The background fluorescence of EVs is indicated by grey histograms.