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. 2017 Aug 15;7:8202. doi: 10.1038/s41598-017-08392-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of extracellular vesicles (EVs) released by ciprofloxacin-exposed control, activated or apoptotic Jurkat cells. (a) Concentration values of EVs in 100 µL (isolated from the supernatant of 2.5 × 10⁷ cells) were determined by tunable resistive pulse sensing (TRPS), and are plotted as a function of their diameter for control, activated or apoptotic cells (blue, green or red colors, respectively). Histogram plots of EVs are representative of three independent experiments. (b) Mode diameters of EV subpopulations were determined by TRPS, and are presented+/− S.D. (error bars) of three independent experiments, for control, activated and apoptotic cells. NP2000, NP800 and NP100 IZON nanopore membranes were used. (c) Transmission electron microscopy images of apoptotic bodies (APOs), microvesicles (MVs) and exosomes (EXOs) samples.