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. 2017 Aug 15;7:8202. doi: 10.1038/s41598-017-08392-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of extracellular vesicles (EVs) derived from ciprofloxacin-exposed control, activated or apoptotic Jurkat cells. EVs were stained by annexinV-FITC and propidium iodide (PI) for flow cytometry and analyzed before and after detergent lysis with 0.1% Triton X-100. Apoptotic bodies (APOs) and microvesicles (MVs) were labeled and measured directly, whereas exosomes (EXOs) were conjugated onto latex beads before staining. Density plots show annexinV-FITC and PI positivity of EVs derived from ciprofloxacin-exposed control, activated and apoptotic Jurkat cells. The percentages of positive events (above the threshold represented by a black line) are shown in the plots.