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. 2017 Aug 15;7:8174. doi: 10.1038/s41598-017-08487-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PYY, GLP-1 and GLP-2 protein expression in human duodenal biopsies. Profile of gut hormones, PYY (A), GLP-1 (B) and GLP-2 (C) was determined in the duodenal biopsies of lean (■), obese () and post-operative obese, (□) by immunohistochemistry. Bar charts (on the right) show number of cells counted expressing the gut hormones. Statistical significance was determined by a One-way ANOVA with differences between means identified using a Holm-Sidak multiple comparison post-test test where *p < 0.05, ***p < 0.001. Scale bars are either 100, 50 or 25 µm. Nuclei are stained blue with 4′,6-diamidino-2-phenylindole (Dapi).

Inline graphic — PYY, GLP-1 and GLP-2 protein expression in human duodenal biopsies. Profile of gut hormones, PYY (A), GLP-1 (B) and GLP-2 (C) was determined in the duodenal biopsies of lean (■), obese () and post-operative obese, (□) by immunohistochemistry. Bar charts (on the right) show number of cells counted expressing the gut hormones. Statistical significance was determined by a One-way ANOVA with differences between means identified using a Holm-Sidak multiple comparison post-test test where *p < 0.05, ***p < 0.001. Scale bars are either 100, 50 or 25 µm. Nuclei are stained blue with 4′,6-diamidino-2-phenylindole (Dapi).