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. 2017 Aug 15;7:8241. doi: 10.1038/s41598-017-08365-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cardiac histopathology of offspring at 12 months of age. Perivascular fibrosis area normalised to lumen area, interstitial fibrosis expressed as percentage of cardiac tissue, and histology score in male (A) and female (B) offspring. (C) Masson’s Trichrome staining of cardiac tissue in males. Blue staining marks collagen (fibrosis). Scale bar represents 200 µm. Scoring analysed via one-way ANOVA with letters denoting statistical differences between groups. Perivascular and interstitial fibrosis analysed via two-way ANOVA. *P < 0.01 by Sidak post hoc. Values are mean ± SEM. Male: N = 5–11/group; female: N = 4–8/group. Control: open bars/points; hypoxia: closed bars/points.