Table 2.
Offspring body and organ weights and renal parameters at 12 months of age.
| Normal Salt | High Salt | Two-way ANOVA | |||||
|---|---|---|---|---|---|---|---|
| Control | Hypoxia | Control | Hypoxia | Treatment | Diet | Inter-action | |
| MALES | |||||||
| Body and organ weights | |||||||
| Bw (g) | 55 ± 2 | 55 ± 2 | 58 ± 3 | 57 ± 2 | n.s. | n.s. | n.s. |
| Kidney (mg) | 724 ± 35 | 715 ± 19 | 841 ± 75 | 912 ± 26 | n.s. | P = 0.002 | n.s. |
| Kidney:bw | 13.2 ± 0.6 | 12.7 ± 0.4 | 14.6 ± 1.3 | 16.2 ± 0.7 | n.s. | P = 0.02 | n.s. |
| Heart (mg) | 253 ± 6 | 245 ± 5 | 273 ± 14 | 268 ± 7 | n.s. | P = 0.007 | n.s. |
| Heart:bw | 4.5 ± 0.1 | 4.5 ± 0.1 | 4.7 ± 0.1 | 4.8 ± 0.2 | n.s. | n.s. | n.s. |
| Renal parameters | |||||||
| Urine output (ml/24 h) | 1.73 ± 0.3 | 1.35 ± 0.3 | 2.01 ± 0.4 | 2.19 ± 0.5 | n.s. | n.s. | n.s. |
| Water consumption (ml/bw/24 h) | 0.11 ± 0.008 | 0.14 ± 0.03 | 0.26 ± 0.05 | 0.19 ± 0.01 | n.s. | P = 0.0003 | n.s. |
| Albumin excretion (µg/24 h) | 9.7 ± 2.1 | 22.4 ± 3.8 | 8.2 ± 2.8 | 27.0 ± 13.0 | P = 0.04 | n.s. | n.s. |
| UNaV (µmol/24 h) | 133 ± 24 | 99 ± 27 | 400 ± 70 | 513 ± 52 | n.s. | P < 0.0001 | n.s. |
| UClV (µmol/24 h) | 154 ± 57 | 130 ± 89 | 374 ± 143 | 513 ± 145* | n.s. | P < 0.0001 | P = 0.04 |
| UKV (µmol/24 h) | 219 ± 23 | 210 ± 41 | 217 ± 37 | 228 ± 39 | n.s. | n.s. | n.s. |
| Plasma cystatin C (ng/mL) | 768 ± 103 | 880 ± 109 | 645 ± 46 | 600 ± 30 | n.s. | P = 0.02 | n.s. |
| FEMALES | |||||||
| Body and organ weights | |||||||
| Bw (g) | 53 ± 2 | 50.8 ± 4 | 54 ± 3 | 49 ± 3 | n.s. | n.s. | n.s. |
| Kidney (mg) | 493 ± 10 | 481 ± 22 | 543 ± 37 | 582 ± 44 | n.s. | P = 0.02 | n.s. |
| Kidney:bw | 9.7 ± 0.7 | 9.1 ± 0.4 | 9.0 ± 0.4 | 10.4 ± 1.0 | n.s. | n.s. | n.s. |
| Heart (mg) | 194 ± 13 | 189 ± 8 | 215 ± 13 | 220 ± 13 | n.s. | P = 0.009 | n.s. |
| Heart:bw | 3.8 ± 0.4 | 3.6 ± 0.2 | 3.6 ± 0.1 | 3.9 ± 0.3 | n.s. | n.s. | n.s. |
| Renal parameters | |||||||
| Urine output (ml/24 h) | 1.50 ± 0.3 | 1.57 ± 0.3 | 2.13 ± 0.3 | 3.02 ± 0.7 | n.s. | n.s. | n.s. |
| Water consumption (ml/bw/24 h) | 0.07 ± 0.005 | 0.074 ± 0.02 | 0.26 ± 0.05 | 0.18 ± 0.07 | n.s. | P = 0.01 | n.s. |
| Albumin excretion (µg/24 h) | 51.5 ± 15.3 | 57.4 ± 18.0 | 39.6 ± 16.5 | 11.8 ± 5.1 | n.s. | n.s. | n.s. |
| UNaV (µmol/24 h) | 111 ± 21 | 76 ± 12 | 534 ± 101 | 479 ± 98 | n.s. | P < 0.0001 | n.s. |
| UClV (µmol/24 h) | 158 ± 27 | 130 ± 21 | 546 ± 95 | 489 ± 92 | n.s. | P < 0.0001 | n.s. |
| UKV (µmol/24 h) | 186 ± 26 | 170 ± 25 | 162 ± 24 | 120 ± 18 | n.s. | n.s. | n.s. |
| Plasma cystatin C (ng/mL) | 1246 ± 270 | 1166 ± 307 | 785 ± 58 | 866 ± 78 | n.s. | P = 0.07 | n.s. |
Values are mean ± SEM. Body weight (bw). Body/organ weights: N = 1–2 offspring from 11 litters/group. Renal parameters: male, N = 6–10/group; female, N = 5–10/group. Effect of treatment, diet or interaction (treatment x diet) was evaluated by two-way ANOVA. *Sidak post hoc comparing control/hypoxia offspring fed the high salt diet. Not significant (n.s.).