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. 2017 Aug 15;7:8290. doi: 10.1038/s41598-017-08197-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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K14 and ∆Np63 double staining of lacrimal glands after ligation-induced injury. Immunofluorescence staining of K14 (green) and ∆Np63 (red) on control lacrimal gland (A), and samples 0 (B), 1 (C), 3 (D), 7 (E), 10 (F), 20 (G) and 30 (H) days post-reopening of the main excretory duct. K14 expression pattern (A–H) and level (I) changed after ligation-induced injury. ∆Np63 positive cell number (J) is significantly higher in D0 to D20 samples. Blue is DAPI staining. D = Day. *P < 0.05. Bar represents 100 μm.