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. 2017 Aug 15;7:8290. doi: 10.1038/s41598-017-08197-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EMT-associated markers in lacrimal gland tissues after duct ligation-induced injury and ligature release. Double staining on control lacrimal gland (A,G), and samples 0 (B,H), 1 (C,I), 3 (D,J), 7 (E,K) and 10 (F,L) days post-reopening of the main excretory duct showed colocalizaion of Vimentin (red, A–L) with epithelia proliferation markers K14 (green, A–F) and ∆Np63 (green, G–L) during tissue repair. Blue is DAPI staining. Bar represents 100 μm. RT-qPCR showed that BMP1 (A), CDH1 (B), SNAI2 (C), and VIM (D) mRNA levels dramatically changed after main secretory duct ligation and reopening (*P < 0.05).