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. 2017 Aug 15;7:8290. doi: 10.1038/s41598-017-08197-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The proliferative capability and characterization of the immature lacrimal gland epithelial cells in monolayer cultured in serum-free media. Monolayer culture of cells from day 7 samples after duct ligature release can form a compactly organized epithelial morphology from passage 0 (P0, A) to P12 (B). Immunostaining showed most of the cells to be nestin positive (green, C), ∆Np63 positive (red, C,F), ABCG2 positive (red, D), PCK positive (green, D) and K15 positive (green, E). Some of these P10 cells to be K14 positive (green, F), RT-qPCR showed the mRNA level of progenitor cell-related genes in the cultured cells (G). Bar represents 100 μm.