Table 1.
Solubility scores, solubility and stability measurements for all mAb variants studied in this work.
| Variant | Solubility measurement | Stability measurement | CamSol intrinsic score (a.u.) | ||||
|---|---|---|---|---|---|---|---|
| PEG1/2 (%) | Apparent solubility (mg/mL) | Tm1 (°C) | TH (°C) | VH | VL | Combined | |
| mAb1 | 4.9 in (4.5,5.1) | 18.8 in (9.7,36.9) | 65.9 | 59.8 | 0.383 | 0.696 | 0.025 |
| mAb2 | 0.4 in (0.32,0.47) | 0.78 in (0.7,0.86) | 55 | 50.6 | −0.304 | 0.708 | −0.465 |
| mAb3 | 0.44 in (0.41,0.48) | 0.89 in (0.78,1.01) | 54.8 | 51.3 | 0.287 | 0.751 | −0.008 |
| mAb4 | 5 in (4.8,5.1) | 29.3 in (12.5,68.3) | 55.3 | 51.5 | 0.42 | 0.645 | 0.017 |
| mAb5 | 6.9 in (6.8,7.2) | 78.2 in (27.6,207.8) | 55.4 | 50 | 0.488 | 0.645 | 0.067 |
| mAb6 | 2 in (1.9,2) | 2.9 in (2.2,3.7) | 54.1 | 48.7 | 0.007 | 0.696 | −0.248 |
| mAb7 | 5.5 in (5.3,5.8) | 23.3 in (5.8,97.1) | 55.3 | 51.6 | 0.38 | 0.775 | 0.076 |
| mAb8 | 4.8 in (4.6,5) | 27.2 in (16.5,44) | 55.5 | 51.5 | 0.282 | 0.557 | −0.142 |
| mAb9 | 5.7 in (5.5,5.8) | 50.6 in (26.9,94.7) | 55.8 | 51.3 | 0.378 | 0.584 | −0.053 |
PEG 1/2 and the apparent solubility are estimated from the fitting of the data points in Figures 3 and S2, respectively. Numbers in brackets are the lower and upper 95% confidence interval calculated with 104 bootstrap cycles. Stability measurements Tm1 and TH have been performed respectively with DSC and DSF (see Methods). The CamSol scores are reported for the sequences of the VH domain, the VL domain, and for the combined VH-VL complex (see Methods).