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. 2017 Aug 15;7:8232. doi: 10.1038/s41598-017-07473-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lep transcript abundance increased at estradiol doses of 0.3 and 1 nM. 3T3-L1 adipocytes were treated for 24 hrs with estradiol doses ranging from 0.3–5 nM. Leptin transcripts were measured by qRT-PCR and normalized to an 18S rRNA control. The transcript abundance relative to untreated controls is reported as mean ± S.E.M of n ≥ 7 biological replicates; *Denotes p-value ≤ 0.05 when compared to the control with 2-sample, independent Student’s t-tests.