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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Nature. 2016 Oct 5;539(7627):89–92. doi: 10.1038/nature19813

Extended Data Figure 1. Absence of antisense transcription 3′ to the Hoxa11 promoter in the Hoxa11eGFP/eGFP limb and evidence that Hoxa11as-b transcripts produced in trans have no effect on Hoxa11 expression.

Extended Data Figure 1

a, b, Detection of Hoxa11as-b transcripts in wild-type limb buds at E12.5 (a), and whole-mount in situ hybridization to detect gfp antisense transcripts in Hoxa11eGFP/eGFP limb buds at E12.5 (b). c–e, Hoxa11 expression in wild-type limb buds (c), and Hoxa11as-b (d) and Hoxa11 (e) expression in Prx1-Hoxa11as limb buds. Original magnification, ×31.5.

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