Skip to main content
. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Nature. 2016 Oct 5;539(7627):89–92. doi: 10.1038/nature19813

Figure 2. Deletion of the distal enhancer in Hoxa11 intron results in impaired antisense transcription and gain of sense transcription in distal cells.

Figure 2

a, Left, scheme of the Tg(m-Inta11-LacZ) transgene carrying the predicted distal enhancer (Int, red box). Right, X-gal staining of E12.5 transgenic embryos (n = 5). B–e, Expression of Hoxa11as-b (b, c) and Hoxa11 (d, e) in wild-type (WT; b, d) and Hoxa11ΔInt/ΔInt (c, e) mouse limbs at E12.5. Note that based on the observed gain of Hoxa11 expression, other regulatory input(s) could be implicated in Hoxa11 regulation in distal cells. Pr, minimal promoter. Original magnification, × 31.5 (for all images).