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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Nature. 2016 Oct 5;539(7627):89–92. doi: 10.1038/nature19813

Figure 4. The mouse Hoxa11 antisense enhancer is functional in distal fins.

Figure 4

a, mVISTA sequence conservation plot of the mouse Hoxa11 intron (red) with tetrapod (rat, human, chicken and frog) and fish representatives (coelacanth and zebrafish). Ex1, exon 1; Ex2, exon 2. Note that zebrafish has two Hoxa11 genes expressed in developing fins, Hoxa11a and Hoxa11b. b, c, GFP expression in fin buds of Tg(m-Inta11-eGFP) transgenic zebrafish embryos at 60 hpf (b) and 72 hpf (c), revealing the enhancer activity of the mouse Hoxa11 intron in fish. Note the filopodia-like protrusions in GFP+ mesenchymal cells suggestive of a migration towards the fin fold. d, e, Hoxa13a expression in developing fins at 60 hpf (d) and 72 hpf (e). Original magnification, ×400.