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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Nature. 2016 Oct 5;539(7627):89–92. doi: 10.1038/nature19813

Extended Data Table 1.

Summary of transient transgenic embryos analysed

Zebrafish Transient Transgenics
Construct % of eGFP positive fish
Tg(HBB:eGFP) 0% (n=74)
Tg(z-Inta11a-eGFP) 0% (n=105)
Tg(z-Inta11b-eGFP) 1.19% (n=84)
Tg(m-Inta11-eGFP) 91.9% (n=123)
Tg(HBB:eGFP; cmlc2:mCherry) 1.25% (n=94)
Tg(z-Inta11a-eGFP; cmlc2:mCherry) 0% (n = 200)
Tg(z-Inta11b-eGFP; cmlc2:mCherry) 0% (n = 300)
Tg(m-Inta11-eGFP; cmlc2:mCherry) 88.9% (n=53)
Mouse Transient Transgenics
Construct % of eGFP positive embryos (# eGFP positive / # genotyped positive)
Tg(z-Inta11a-eGFP) 0% (n=0/10)
Tg(z-Inta11b-eGFP) 0% (n=0/7)

Zebrafish stable lines for Tg(z-Inta11a-eGFP; cmlc2:mCherry); Tg(z-Inta11b-eGFP; cmlc2:mCherry) were also generated and three genotyped Fi embryos per line were analysed and confirmed for the absence of gfp expression. For Tg(m-Inta11-eGFP; cmlc2:mCherry), four distinct transgenic lines were also generated and analysed.