Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 3;36(32):4641–4652. doi: 10.1038/onc.2017.91

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

PMC Copyright notice

Noncanonical activation of GLI1 in LAC cells. (a, b) Western blots of endogenous protein levels of NRP2, GLI1 and P-ERK1/2 and actin (LC) in commercial LAC cell lines (H1734, H1437) and CSCs 2, 3, 4 after siRNA-mediated (a) or short-hairpin-mediated (b) silencing of NRP2. Bar graphs: densitometric analyses. Dashed line: CTRL treatment. (c–f) Cell viability (MTS assay) in (c) LAC cell lines after siRNA silencing of NRP2, GLI1 and both; in (d) CSCs after short-hairpin mediated silencing of NRP2, GLI1 and both; and in (e) in LAC cell lines and (f) LAC CSCs treated for 48 h with GANT61, U0126 or both. *P<0.05, **P<0.01 vs sham-treated/sham-transfected controls.