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. 2017 Apr 3;36(32):4551–4561. doi: 10.1038/onc.2017.89

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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circRNA_100290 serves as a sponge for the miR-29 family. (a) A schematic model showing the putative binding sites for miRNAs and 3′UTR of circRNA_100290. (b) Luciferase reporter assay revealed that miR-29b, miR-29a, miR-29c and miR-299 were able to reduce the luciferase intensity more than 40%. (c) Binding sites of miR-29b in 3′UTR of circRNA_100290. (d, e) miR-29b or negative control oligonucleotide was co-transfected with pmiR-RB-Report vector with or without the 3′UTR sequence of circRNA_100290. The RLU of hRluc and hLuc+ were determined, and the hRluc values were normalized to the corresponding hLuc+ values, **P<0.01. (f) The relative expression levels of circRNA_100290 and miR-29b in CAL27 cell lines. ΔCt values were used to measure gene expression, which was normalized by GAPDH expression levels. The presented values are the means±SD.