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. 2017 Aug 14;8:1403. doi: 10.3389/fpls.2017.01403

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Copyright © 2017 Lang, Deng, Zhao, Deng, Zhang, Zhang, Zhang, Sa, Yao, Wu, Wu, Deng, Lin, Xia and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Na⁺ concentrations in root and leaf cells of NaCl-stressed Glycyrrhiza uralensis. Two-week-old G. uralensis seedlings were exposed to 0 or 100 mM NaCl for 6, 12, and 24 h in one-quarter-strength Hoagland solution, then stained with CoroNa-Green AM to detect Na⁺ concentrations. Representative confocal images of (A,B) roots (scale bar: 250 μm) and (C,D) leaves (scale bar: 50 μm) show the Na⁺ content (bright green fluorescence). The orange-red color is chlorophyll autofluorescence. The mean value (±SD) of 4–5 independent experiments is shown in the left bottom corner of each panel, and different letters (a and b) denote significant differences (P < 0.05) between control (–NaCl) and salt treatment (+NaCl).