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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Clin Cancer Res. 2013 Apr 24;19(12):3176–3188. doi: 10.1158/1078-0432.CCR-13-0042

Figure 5.

Figure 5

Systemic infusion of M2 macrophages and blocking IL-17 activity suppress BRONJ-like lesion formation in MM mice. A, Treatment regimen. Mice were intravenously given one dose of zoledronate (Zol) (125μg/kg) one week before tooth extraction followed by intravenous injection of Zol twice a week for two weeks. One day after tooth extraction, mice were adoptively transferred with ex vivo expanded M2 macrophages (1x106) or administered with laquinimod (Laq; 5mg/kg) or IL-17 neutralizing antibody (@IL-17, 0.3mg/mice). B–D, Amelioration of BRONJ-like lesion formation by adoptive transfer of ex vivo expanded M2 macrophages, daily oral administration of Laq or intraperitoneal (i.p.) administration of murine neutralizing @IL-17 one day after tooth extraction. Data represent mean ± SEM (n=10 per group). **P<0.01 as compared with Ext control; &P < 0.05 and &&P<0.01 as compared with Zol (+). E, A reduction in serum IL-17 level after adoptive transfer of ex vivo expanded M2 macrophages, daily oral administration of Laq or intraperitoneal (i.p.) administration of murine neutralizing @IL-17 one day after tooth extraction. Data represent mean ± SEM (n=5 per group). ##P<0.01 as compared with C57BL/6 mice (B6); **P<0.01 as compared with Ext control; &P < 0.05 and &&P<0.01 as compared with Zol (+). F and G, A decrease in M1/M2 ratio in mucosal tissues bordering extraction sockets of mice after adoptive transfer of ex vivo expanded M2 macrophages, daily oral administration of Laq or intraperitoneal (i. p.) administration of murine neutralizing @IL-17 one day after tooth extraction. Data represent mean ± SEM of quantification of M1/M2 macrophages in BRONJ-like lesions of mice (multiple images, n=3 per group). #P<0.05 and ##P<0.01 as compared with C57BL/6 mice (B6); $P<0.05 as compared with Ext control; *P<0.05 and **P<0.01 as compared with Zol (+) BRONJ (−); &P < 0.05 and &&P<0.01 as compared with Zol(+)BRONJ(+).