Fig. 4.

Description of the pipeline for determining multi-protein RAS complexes. Protein interactions in STRING were manually evaluated and PDB structures were acquired. PRISM was used to predict complex formation. These complexes were combined with available RAS complexes in the PDB to gain a complete view of RAS binding surfaces. This was used to determine which binding partners could bind simultaneously. Simultaneous binding was then assessed in a 3D model to ensure that no steric clashes resulted