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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Methods Mol Biol. 2017;1619:319–337. doi: 10.1007/978-1-4939-7057-5_22

Figure 2.

Figure 2

Results of the second pilot ratio check for a study involving six TMT10plex experimental sets. The total intensity of each reporter ion channel is shown after 1 to 5 μL of each differentially tagged sample were combined and analyzed in a single LC- MS/MS run. The volumes combined were adjusted based upon the data in Figure 1. Reporter ions are color-coded from lowest (yellow=126 Da) to highest mass from left to right respectively for each experimental set.