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. 2017 Aug 1;130(15):2520–2529. doi: 10.1242/jcs.201111

Fig. 5.

Fig. 5.

Retrograde transport is not required for DCV distribution in type II terminals. (A) ANF–GFP with expression of a dominant-negative form of the dynactin Glued subunit (UAS-GlΔ96) in type Ib processes on m2 labeled with elav–GAL4. The membrane is labeled with TRITC-HRP antibody. Asterisk indicates the most distal bouton. Scale bar: 10 µm. (B,C) Quantification of the total number of boutons of type I (Ib+Is) (B) and type II (C) terminals on each muscle in the wild type and in GlΔ96 overexpression larvae based on anti-HRP labeling. The number on each bar represents how many endings were analyzed from 8 animals for type I boutons and 10 animals for type II boutons. Student's t-test was used to calculate statistical significance; *P<0.05, ****P<0.0001. (D) ANF–GFP in type II boutons on m2 with expression of GlΔ96. The asterisk indicates the most distal bouton. Scale bar: 10 µm. (E) Quantification of the ratio of fluorescence intensity of distal boutons to proximal boutons. Total number of m2 endings examined derived from 10 animals is shown for each group. Student's t-test was used to calculate the P-value. *P<0.05.