Fig. 2.

Anodic localization of HS in the presence of an EF. The distribution of HS was measured by performing a maximum intensity projection of confocal z-stacks of individual cells to minimize any error associated with out-of-focus pixels. In the absence of an EF, HS is distributed uniformly across the membranes of fNPCs (A), astrocytes (C) and BTICs (E). However, strong anodic localization of HS was observed across all three cell lines (B,D,F) in the presence of an EF despite fNPCs and BTICs migrating towards the cathode and astrocytes migrating towards the anode. (G) Distribution of HS was analyzed by performing a signed rank test among all the pixels in the cathode face (c) versus pixels in the anode face (a). (H) Percentage of cells with anodic localization of HS is higher in the presence of an EF than no EF among all three cell types. Each condition represents more than 50 cells. White arrows in panels C and D indicate the ECM left behind by cells during migration.