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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Curr Biol. 2014 Dec 4;25(1):124–130. doi: 10.1016/j.cub.2014.11.041

Figure 1. Pix and Git physically interact with Hippo and regulate Hippo pathway activity.

Figure 1

For co-immunoprecipitation experiments, protein lysates from S2 cells transfected with the indicated plasmids (A) or wing imaginal discs expressing the indicated transgenes (B) were incubated with anti-Flag or anti-HA antibodies, respectively. Western blot analysis was performed using anti-Flag, anti-HA and anti-GFP antibodies to reveal Hpo, Pix and Git, respectively. (C and D) D. melanogaster eyes 44 hours after puparium formation stained with anti-Discs-large. Eyes express UAS-Yki-S168A-YFP under the control of GMR-Gal4, either alone (C) or together with UAS-Pix RNAi (D). (E, E′) D. melanogaster third instar larval wing imaginal disc of the genotype: ex-lacZ, en-Gal4, UAS-GFP; UAS-Pix RNAi. Transcriptional activity of the ex gene was reported by β-galactosidase expression(grayscale) and in (E′) GFP (green) demarcates the posterior compartment, where Pix RNAi was expressed. (F–H′) Adult female D. melanogaster eyes, dorsal views in (F, G and H), lateral views in (F′, G′ and H′). Eyes express UAS-Yki-S168A-YFP under the control of GMR-Gal4, either alone (F, F′) with UAS-Pix RNAi (G, G′), or with UAS-Pix RNAi and UAS-Sd RNAi (H, H′). See also Figure S1.