Fig. 8.

Analysis of signaling events in RIG-I-activated GEN2.2 cells in the presence or absence of AMA. Cytosolic RIG-I expression was induced in GEN2.2 by pre-treatment with CpG-A (0.25 μM) for 16 h then cells were stimulated with the specific RIG-I ligand 5′ppp-dsRNA (RIGL, 1 μg/ml) in the presence or absence of AMA (0.5 μg/ml). After 2 h of stimulation the expression of the MAVS adaptor protein (A, B) and after 30 min of stimulation the phosphorylation of IRF3 (C, D) and Akt (E, F) were determined by Western blotting. Representative blots (A, C, E) and the means ± SD of four independent experiments (B, D, F) are shown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control, ^#p < 0.05, ^##p < 0.01 vs. RIGL. AMA: Antimycin-A.