Table 1. . Predicted cellular localization of glutaminase isozymes.
| Algorithm |
Isoform |
Ref. | |||
|---|---|---|---|---|---|
| KGA | GAC | LGA_long | LGA_short | ||
| Hum-mPLoc 2.0 |
Mitochondria |
Mitochondria |
Mitochondria |
Mitochondria |
[41] |
| TargetP 1.1 |
Mitochondria |
Mitochondria |
Mitochondria |
Not mitochondria |
[42] |
| MultiLoc2 |
Mitochondria |
Mitochondria |
Mitochondria |
Cytosol |
[43] |
| SLCPred |
Cytosol |
Cytosol |
Cytosol |
Cytosol |
[44] |
| iLoc-Animal | Cytosol | Mitochondria | Cytosol | Cytosol | [45] |
Five cellular localization algorithms were queried with the full sequence of each major glutaminase isoform. Only Hum-mPloc 2.0 predicted that all four would localize to the mitochondria, and only SLCPred predicted that all four would localize to the cytosol.
GAC: Glutaminase; KGA: Kidney-type glutaminase; LGA: Liver-type glutaminase.