Skip to main content
. 2017 Jul 6;7(10):2673–2689. doi: 10.7150/thno.18915

Figure 1.

Figure 1

Characterization and validation of senescent MSCs. (A) The morphology of MSCs observed under an inverted microscope at passage 4, 13, 17, and 22 (P4, 13, 17, and 22). (×100; arrows indicate senescent cells). (B) Immunophenotypic analysis of surface markers CD29, CD44, CD73, CD90 and CD105 in MSCs at P5, 14, and 22 by FCM. (C) β-gal staining (blue) of MSCs observed under an inverted microscope at P5, 14, 17, and 22. (×100; arrows indicate senescent cells). (D) Cell cycle characteristics of different passage MSCs (P5, 13, and 22) by FCM. (E) RT-PCR demonstrating mRNA expression levels of p16 and p21, n = 4. (F) ELISA analysis of IL-6, IGFBP4, IGFBP7, and MCP-1 in different passage MSCs (P5, 15, and 20). n = 5. (G-H) Oil Red O staining (G) and Alizarinl Red S staining of different passage MSCs (P5, 18) observed under an inverted microscope at 2 weeks. (×200). Each experiment was confirmed in four different donors, and the representative data are shown. Abbreviations: AIM: adipogenic induction medium; CM: complete medium; AIM: adipogenic induction medium; OIM: osteogenic induction medium.