A, Temperature profile of a 3.5min continuous FUS+HT to 42°C group and B, the subsequent release of DOX (n=3). C, A comparison of the sum of each discrete AUC measurement in the 10x 30s of FUS+HT and unheated controls as well as the continuous AUC measured from the 3.5min continuous FUS+HT group. There is no significance between the intravascular AUC in the short duration and 3.5min heating group. However, there is a significant difference in the extravascular compartment. Significance was evaluated using one-way ANOVA following by a multiple comparison test in Matlab between the 30s FUS+HT and unheated controls at each time point, (*) p < 0.05, (**) p < 0.01, (†) p < 0.001.