Fig 2.

Identification of apoptosis-inducing proteins in NK and NK-92 Exo. Fifty micrograms of total proteins from NK cell and NK-92 Exo lysates were loaded into each lane (n = 3). (A) and (B) The apoptosis-inducing proteins FasL and perforin were expressed in both NK cell and NK-92 Exo lysates. FasL was also detected in a membrane proteins enriched extract of NK-92 Exo. (C) and (D) The abundances of FasL and perforin in NK and NK-92 Exo were estimated using the software EvolutionCapt. NK-92 Exo lysates contained higher abundances of FasL and perforin than the NK cell lysates did. Experiments were performed at least in triplicate and values ± SD were plotted. *p < 0.05.