V235fs and D272fs mice exhibit altered peritoneal B-1 and B-2 population. (A) FACS analysis of major B and T cell populations in the spleen. Top, gating strategy for follicle (FoB) and marginal zone (MZB) B cells. Gating strategy for CD4 and CD8 T cells is not shown. Bottom, quantitation of each cell population (as indicated) from WT, V235fs and D272fs mice. (B) FACS analysis of peritoneal B-1 and B-2 cells. Gating strategy on the left and quantitation on the right. *, p<0.05. Error bar, SEM. Data are representative of at least two independent experiments.