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Representative fluorescence quenching experiments of CQ binding to sapB in 20 mM phosphate buffer, pH 7.4. Conditions: several 2 to 3 μL injections of [CQ] = 1.0 mM solution into the fluorescence cuvette containing [sapB] = 40 μM. λex = 280 nm and λem = 300 – 450 nm with excitation and emission slits opening of 10 nm each.
