Figure 2.

Insufficient B10 aggravated the Th1 response in the Teff and B cell coculture system in vitro. (a1, a2) The percentages of CD4⁺IFN-γ⁺ Th1 cells in different groups of the B cell and Teff coculture system were studied by flow cytometry. (b, c) The expressions of IFN-γ and T-bet in different groups of the B cell and Teff coculture system with or without insert plates were assayed by real-time PCR. (d) The secretions of IFN-γ in supernatant of the B cell and Teff coculture system were assayed by CBA. (e, f) The concentrations of IFN-γ and IL-2 in supernatant of the B cell and Teff coculture system with insert plates were assayed by CBA. (Data was presented as mean ± SEM (n = 5). ^∗p < 0.05, significantly different compared with the control B + Teff group. ^#p < 0.05, significantly different compared with the silica B + Teff group.)