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. 2017 Aug 2;2017:8415094. doi: 10.1155/2017/8415094

Figure 4.

Figure 4

Insufficient B10 both suppressed Treg and inhibited the conversion of Teff into Treg. (a1, a2) The percentages of CD4+CD25+ Treg in different groups of the B cell and Treg coculture system were studied by flow cytometry. (b–e) The expressions of Foxp3, CTLA4, IL-10, and TGF-β in different groups of the B cell and Treg coculture system were assayed by real-time PCR. (f1, f2) The percentages of CD4+CD25+ Treg in different groups of the B cell and Teff coculture system were studied by flow cytometry. (g–j) The expressions of Foxp3, CTLA4, IL-10, and TGF-β in different groups of the B cell and Teff coculture system with or without insert plates were assayed by real-time PCR. (k, l) The secretions of IL-10 in supernatant of the B cell and Teff coculture system were assayed by CBA. (Data was presented as mean ± SEM (n = 5). ∗p < 0.05, significantly different compared with the control B + Teff group. #p < 0.05, significantly different compared with the silica B + Teff group.)