Fig. 3.

Fractalkine prevents oxidative stress-induced demyelination in cerebellar slice cultures. Organotypic cerebellar slices were pre-treated with fractalkine (CX3CL1; 100,10 or 1 ng/ml) for 1 h prior to treatment with glucose oxidase (GOX) and catalase (CAT); concentrations were maintained constant at 1:100,000 and 1:500,000, respectively, to generate low levels of H₂O₂ continuously. a Images are representative of fractalkine (CX3CL1) at 100 ng/ml treatment group. Confocal images taken at × 10 magnification. Scale bar, 100 μm. Molecular layer (ML) and white matter tract (WMT) labelled. b Graph shows a significant decrease in MBP fluorescence with GOX-CAT treatment. This effect is rescued with fractalkine treatment at 100 ng/ml but not at c 10 ng/ml or d 1 ng/ml. NFH shows no significant decrease in fluorescence with GOX-CAT treatment (e–g). Graphs expressed as mean ± SEM; Student’s t test, ^### p < 0.001; and one-way ANOVA, **p < 0.01; n = 5