Figure 7.

Effect of KPE on mitochondrial biogenesis transcription factor expression in vitro. Hs68 cells were pretreated with KPE (1–10 μg/ml) or resveratrol (10 μM) for 24 h in a serum-free medium. After 2 h of H₂O₂ exposure, the cells were cultured for additional 24 h. (a) Transcriptional changes in PGC-1α, ERRα, NRF-1, and Tfam were detected via RT-PCR. (b) Protein levels of PGC-1α were evaluated via western blotting. GAPDH and α-tubulin were used as the mRNA and protein loading controls, respectively.