Figure 6.

miR-646 suppresses the EMT via Akt/mTOR signalling. (A) ISH analysis of miR-646 and IHC analysis of FOXK1 and E-cadherin were performed. These figures were the representatives of gastric tissues from 10 cancerous and 10 non-cancerous patients. The scale bars represent 200 μm in A. (B) Knockdown of FOXK1 by siRNA in gastric cancer cell decreased the protein expression of E-cadherin, vimentin and phosphorylation of Akt and mTOR. (C) FOXK1, mTOR, p-mTOR, Akt, and p-Akt protein expression in BGC-823 and AGS cells transfected with the miR-646 mimic, inhibitor, or corresponding control (NC) plasmid.