Figure 5.

ASM inhibitor-mediated cell death involves disruption of MMP but not Caspase-3/7 activity. (A) flow cytometry analysis showing Annexin-V/PI staining of UACC 903 cells. Cells, in the lower and upper right quadrant show early and late apoptotic cells, respectively; (B) histogram showing MMP following treatment with ASM inhibitors or FCCP; (C) viability of wild-type or BAX-knockout HCT116 cells after 24 h of treatment with increasing concentrations of perphenazine (left) or other ASM inhibitors (right); (D) viability of UACC 903 cells treated with leelamine or ASM inhibitors in the absence or presence of apoptosome inhibitor NS3694; (E) caspase-dependent cell death measured by treatment of UACC 903 cells with or without pan-caspase inhibitor, z-VAD-fmk. TRAIL (50 ng ml⁻¹) treatment served as a positive control for caspase-dependent cell death (left). *P < 0.05; **P < 0.01; ***P < 0.001.