Table 1. Geneset enrichment analysis.
| Pathway | P-value | Z-score | Tool |
|---|---|---|---|
|
(A) RNA-Seq enrichment analysis | |||
| Regulation of cholesterol biosynthesis by SREBF | 3.10E−10 | −2.05 | Reactome |
| Interferon α/β-signalling | 4.10E−06 | −1.93 | Reactome |
| Cholesterol biosynthetic process | 1.09E−11 | −2.42 | GOBP |
| Cholesterol metabolic process | 1.87E−09 | −2.21 | GOBP |
| Response to decreased oxygen levels | 3.78E−08 | −2.48 | GOBP |
| Response to nutrient levels | 1.75E−06 | −2.4 | GOBP |
| Response to endoplasmic reticulum stress | 2.03E−05 | −2.18 | GOBP |
| Endoplasmic reticulum membrane | 2.62E−04 | −2.29 | GOCC |
| Perinuclear region of cytoplasm | 3.86E−03 | −2.3 | GOCC |
| SREBF1 | 3.41E−08 | −1.88 | ENCODE |
|
(B) RNA-Seq groupwise comparison enrichment analysis | |||
| Cholesterol biosynthetic process | 7.95E−13 | −2.41 | GOBP |
| Cholesterol metabolic process | 5.49E−13 | −2.21 | GOBP |
| Steroid biosynthetic process | 1.78E−07 | −2.22 | GOBP |
| ER-nucleus signalling pathway | 3.00E−07 | −2.19 | GOBP |
| Cellular response to hypoxia | 4.97E−07 | −2.24 | GOBP |
| Response to nutrient levels | 2.04E−06 | −2.42 | GOBP |
| Isoprenoid biosynthetic process | 8.76E−06 | −2.71 | GOBP |
| Response to endoplasmic reticulum stress | 3.79E−06 | −2.22 | GOBP |
| Regulation of cholesterol biosynthesis by SREBP | 3.12E−09 | −2.05 | Reactome |
| Activation of gene expression by SREBF (SREBP) | 1.40E−05 | −2.06 | Reactome |
| UPR | 2.15E−04 | −1.93 | Reactome |
|
(C) Microarray pairwise comparison enrichment analysis | |||
| Regulation of cholesterol biosynthesis by SREBP | 3.65E−07 | −2.05 | Reactome |
| PERK regulates gene expression (ER stress) | 5.16E−06 | −2.14 | Reactome |
| UPR | 4.33E−06 | −1.94 | Reactome |
| Cholesterol biosynthetic process | 1.19E−10 | −2.42 | GOBP |
| Response to nutrient levels | 3.27E−09 | −2.45 | GOBP |
| Response to endoplasmic reticulum stress | 2.13E−09 | −2.25 | GOBP |
| Cholesterol metabolic process | 1.85E−09 | −2.2 | GOBP |
| Endoplasmic reticulum membrane | 1.02E−07 | −2.29 | GOCC |
| Perinuclear region of cytoplasm | 6.27E−03 | −2.28 | GOCC |
|
(D) Microarray groupwise comparison enrichment analysis | |||
| Regulation of cholesterol biosynthesis by SREBP | 1.91E−12 | −2.05 | REACTOME |
| UPR | 2.04E−10 | −1.94 | REACTOME |
| PERK regulates gene expression (ER stress) | 7.94E−08 | −2.12 | REACTOME |
| Cholesterol biosynthetic process | 5.49E−16 | −2.42 | GOBP |
| Response to endoplasmic reticulum stress | 2.24E−14 | −2.25 | GOBP |
| Cholesterol metabolic process | 3.30E−12 | −2.2 | GOBP |
| ER-nucleus signalling pathway | 7.01E−12 | −2.2 | GOBP |
| Autophagy | 7.75E−05 | −2.09 | GOBP |
| ER to Golgi vesicle-mediated transport | 1.84E−04 | −1.92 | GOBP |
| Response to starvation | 2.70E−07 | −2.19 | GOBP |
| Intrinsic apoptotic signalling pathway in response to ER stress | 2.15E−06 | −2.36 | GOBP |
Abbreviations: ER=endoplasmic reticulum; GOBP=Gene Ontology Biological Process, GOCC=Gene Ontology Cellular Component; UPR=unfolded protein response.