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. 2017 Aug 2;2017:2786427. doi: 10.1155/2017/2786427

Figure 2.

Figure 2

Influence of intrathecal administration of TNF and TNF + RTX on thermal hyperalgesia. (a) Administration of NaCl or RTX did not affect paw withdrawal thresholds, compared to control conditions (p > 0.05). (b) Two nanograms of TNF did not lead to changes in paw withdrawal thresholds 1 h, 3 h, 6 h, or 24 h after administration (p > 0.05). Additional administration of RTX (1.9 mg/kg) resulted in changes in withdrawal threshold over the experimental time. When TNF + RTX were administered, a significant increase of withdrawal threshold was observed after 3 (p < 0.05), 6 (p < 0.05), and 24 h (p < 0.05), compared to TNF administration alone. (c) Administration of TNF did not change paw withdrawal thresholds over the experimental time, compared to pretesting (p > 0.05). When RTX was additionally administered, paw withdrawal threshold was significantly increased (p < 0.05). When TNF + RTX was compared to TNF administration alone, an increase of withdrawal threshold was observed after 1 h (p < 0.05), 3 h (p < 0.05), 6 h (p < 0.05), and 24 h (p < 0.05). (d) Administration of 200 ng TNF resulted in a reduction of paw withdrawal threshold after 24 h (p < 0.05), while no changes were observed after 1 h, 3 h, or 6 h (p > 0.05). Additional RTX led to significant increase of paw withdrawal threshold 24 h after administration (p < 0.05). Additional RTX abolished the TNF-induced reduction of paw withdrawal threshold 24 h after administration (∗p < 0.05).