Fig. 2.

The absence of CX3CR1 leads to a decrease in Tau internalization by microglia in vivo. Representative images of the dentate gyrus (DG) of WT (a-b) and KO mice (c-d) stereotaxically injected with PBS-Cy5 or Tau-Cy5 and sacrificed one week later. Immunofluorescence images for Iba1 (green) and Cy5 (red) and high-power magnification are shown separately. e Colocalization between Iba1 and Cy5 in mice injected with PBS-Cy5 or Tau-Cy5 in the DG. Reduced colocalization between Cy5 and Iba1 can be observed in KO animals injected with Tau-Cy5 in comparison to WT mice that received the same injection. f Quantification of Cy5 fluorescence intensity in mice injected with PBS-Cy5 or Tau-Cy5. An increased level of Cy5 fluorescence intensity can be observed in KO mice injected with Tau-Cy5 in comparison to WT mice that received the same injection. Bars show means ± S.E. ∗p ≤ 0.05; ∗∗p ≤ 0.01; + 0.05 < p ≤ 0.1. Purple scale bar: 100 μm, Blue scale bar: 50 μm. GL, granule layer; H, hilus; ML, molecular layer; CA3, cornu ammonis region 3; DG, dentate gyrus