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. 2017 Aug 15;8:27. doi: 10.1186/s13293-017-0149-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Robust argonaute (Ago) footprints are present in the mRNAs of genes that mediate steroid signaling in the neonatal (PN2) hypothalamus. The distribution of Ago footprints in Esr1 (a), Esr2 (b), Ar (c), Pgr (d), and Cyp19a1 (e) are shown. The vertical bars in the first three tracks indicate robust Ago footprints (footscore > 621, BC ≥ 2) in F/Veh, M/Form, or M/Veh groups. 5′ and 3′ UTRs annotated in GenBank RefSeq RNA features are indicated in purple. The majority of Ago footprints aligned to the target mRNA’s 3′ UTR. A stacked height profile (SHP) indicates the read counts of overlapping oligos that aligned to the designated mRNA in the M/Veh group