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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Biomaterials. 2017 Mar 30;131:111–120. doi: 10.1016/j.biomaterials.2017.03.039

Figure 2. Materials characterization and hiPSC-CM viability.

Figure 2

a 1H-NMR data confirms that gelatin chains were functionalized with VS at three different degrees (low, intermediate, and high) as indicated by integral area under the peak between 6.0–6.9 ppm (orange box). The peak between 7.0–7.3 ppm was used as gelatin loading control (blue box). b in vitro degradation assay revealed that low VS-functionalized gelatin hydrogels led to the fastest hydrogel degradation (•), while high VS-functionalized gels led to the slowest degradation (■). c Unconfined compression test showed that increasing degree of VS functionalization led to increasing compressive modulus of gelatin hydrogels. d Live-Dead assay demonstrated relatively high cell viability in all three study groups over the 14-day 3D culture period. (scale bar: 100 μm) e AlamarBlue assay confirmed that hiPSC-CM metabolic activity in 3D hydrogels were not significantly different from that of the 2D control group at day 1 (one-way ANOVA, n.s. p>0.05).