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. 2017 Sep;23(9):1385–1392. doi: 10.1261/rna.060723.117

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© 2017 Silva et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 1. — Mouse Nef-sp is exclusively expressed in the testes. (A) Nef-sp transcript is detected in total RNA from human and mouse testes using reverse transcription PCR (RT-PCR). Testis-specific human PIWIL2 or mouse Piwil2/Mili and ubiquitously expressed Gapdh serve as controls. (B) Quality of total RNA isolated from human cell cultures (HeLa and HEK293) and different mouse tissues is indicated by ethidium bromide staining to reveal the 28S and 18S rRNAs (top panel). RT-PCR analysis of Nef-sp, Mili, and Actin B in the different RNA samples (lower panel). Reactions carried out without (−) and with (+) reverse transcriptase (RT) are shown. (C) Domain organization of human NEF-sp (hNEF-sp) showing the nuclease domain and two RNA-recognition motifs (RRMs). The conserved residues DEDDh (in boxes) distributed in three motifs, and the point mutation (D313A) introduced in motif III, are indicated.