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. 2017 Sep;23(9):1385–1392. doi: 10.1261/rna.060723.117

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© 2017 Silva et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — Human NEF-sp localizes to the nucleolus, while mouse NEF-sp is nuclear in human cell cultures. (A) Cartoon showing domain organization of hNEF-sp. The nuclear localization signal (NLS) is indicated. The predicted NLS sequences are shown below and amino acid (aa) boundaries are indicated. Only the amino-terminal NLS is experimentally proven to be functional. (B,C) Immunofluorescence analyses of HA-tagged proteins transiently expressed in human HeLa cell cultures. The signal from detection of the HA-tagged protein (green) and DNA with DAPI staining (blue) is shown. A merged image is provided. (D) Immunofluorescence detection in transfected mouse NIH3T3 cells. Scale bar in micrometers is shown.