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. 2017 Sep;23(9):1404–1418. doi: 10.1261/rna.061556.117

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© 2017 Chen et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Effects of Pan3 domain truncations on Pan3–PABP and Pan3–Pan2 interactions. (A) Schematic diagram showing various domain truncations of Pan3S and Pan3L. (B,C) Coimmunoprecipitation (Co-IP) and Western blot analyses showing the effects of Pan3 domain truncations on HA-Pan3 isoforms’ abilities to pull down endogenous PABP and Pan2. NIH3T3 cells were transfected with plasmids coding for the indicated epitope-tagged proteins. GAPDH served as the negative control for co-IP. The amount of each “Input” sample loaded onto the gel was 5% of the amount of the corresponding lysate used for IP.