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. 2017 Sep;23(9):1432–1443. doi: 10.1261/rna.061259.117

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© 2017 Moriggi et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 5. — The extranucleolar preribosome body is enriched in maturing complexes primed for nuclear export. (A) Scheme of the experimental plan used to analyze the formation and maintenance of the extranucleolar preribosome body upon leptomycin B (LMB)-mediated Crm1 inactivation in crm1-T539C cells. HU, hydroxyurea. (B) Quantification of cells with GFP-Rrp12 localized at the extranucleolar body upon release from HU arrest under the indicated experimental conditions (inset). (C) Quantification of the percentage of GFP-Tsr1 and GFP-Nop7 localized at the extranucleolar body upon release from HU arrest for 40 min in the presence and absence of LMB.