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Degradation of rRNA during extended stationary phase. WT, RNase II−, RNase PH−, and RNase II− RNase PH− cells were grown in 50 mL M9/glucose at either 31°C or 42°C, and sample volumes corresponding to an equal number of cells were taken every 24 h. Cells were collected by centrifugation, and total RNA was extracted and resolved by Synergel/agarose gel electrophoresis, as described in Materials and Methods. The gel was stained with ethidium bromide and visualized by UV.
